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genome bisulfite sequencing  (Novogene)

 
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    Structured Review

    Novogene genome bisulfite sequencing
    Samples taken from the two MA pedigrees for different experiments. A) Ten MA lines were sampled from the two pedigrees. Samples for <t>sequencing</t> were taken after 5, 20, and 40 transfers and DNA methylation was detected using bisulfite sequencing (red circles). B) Three additional MA lines were sampled from both pedigrees and samples were taken after 1, 5, 7, 8, 10, and 15 transfers for Nanopore sequencing and after 5, 8, 10, 20, and 40 transfers for H3K9me3 ChIP-seq.
    Genome Bisulfite Sequencing, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/genome+bisulfite+sequencing/genome+sequencing+whole/bio_rxiv__64898__2026__03__03__709258-209-2-10
    Average 86 stars, based on 1 article reviews
    genome bisulfite sequencing - by Bioz Stars, 2026-10
    86/100 stars

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    1) Product Images from "Centromeres are hotspots of cytosine methylation epimutations in a filamentous fungus"

    Article Title: Centromeres are hotspots of cytosine methylation epimutations in a filamentous fungus

    Journal: bioRxiv

    doi: 10.64898/2026.03.03.709258

    Samples taken from the two MA pedigrees for different experiments. A) Ten MA lines were sampled from the two pedigrees. Samples for sequencing were taken after 5, 20, and 40 transfers and DNA methylation was detected using bisulfite sequencing (red circles). B) Three additional MA lines were sampled from both pedigrees and samples were taken after 1, 5, 7, 8, 10, and 15 transfers for Nanopore sequencing and after 5, 8, 10, 20, and 40 transfers for H3K9me3 ChIP-seq.
    Figure Legend Snippet: Samples taken from the two MA pedigrees for different experiments. A) Ten MA lines were sampled from the two pedigrees. Samples for sequencing were taken after 5, 20, and 40 transfers and DNA methylation was detected using bisulfite sequencing (red circles). B) Three additional MA lines were sampled from both pedigrees and samples were taken after 1, 5, 7, 8, 10, and 15 transfers for Nanopore sequencing and after 5, 8, 10, 20, and 40 transfers for H3K9me3 ChIP-seq.

    Techniques Used: Sequencing, DNA Methylation Assay, Methylation Sequencing, Nanopore Sequencing, ChIP-sequencing

    Pairwise divergence in cytosine methylation patterns among the MA lines and their ancestors. X-axis shows the number of mitoses separating the two lines that are being compared, and the y-axis shows cytosine methylation divergence. Red line shows a neutral epimutation accumulation model fitted to all data, orange line shows a neutral epimutation accumulation model fitted to data with outlier samples removed, and solid blue line shows a logistic model fitted to all data, and the dotted line shows null model of no increase in divergence. A) The divergence calculated from single cytosines, for CG, CHG, and CHH sequence motifs. B) The divergence calculated for DMRs in different sequence contexts, as in panel A.
    Figure Legend Snippet: Pairwise divergence in cytosine methylation patterns among the MA lines and their ancestors. X-axis shows the number of mitoses separating the two lines that are being compared, and the y-axis shows cytosine methylation divergence. Red line shows a neutral epimutation accumulation model fitted to all data, orange line shows a neutral epimutation accumulation model fitted to data with outlier samples removed, and solid blue line shows a logistic model fitted to all data, and the dotted line shows null model of no increase in divergence. A) The divergence calculated from single cytosines, for CG, CHG, and CHH sequence motifs. B) The divergence calculated for DMRs in different sequence contexts, as in panel A.

    Techniques Used: Methylation, Sequencing

    Related Articles

    Methylation Sequencing:

    Article Title: Mutant IDH1 inhibition induces dsDNA sensing to activate tumor immunity
    Article Snippet: .. The total genomic DNA was submitted to Novogene for whole genome bisulfite sequencing. .. The library preparation for this sequencing utilized the EZ DNA Methylation Gold Kit (Zymo Research) for DNA bisulfite conversion and the Accel-NGS ® Methyl-Seq DNA Library Kit for library construction.

    Article Title: TIGAR deficiency enhances cardiac resilience through epigenetic programming of Parkin expression.
    Article Snippet: .. These samples were submitted to Novogene for whole genome bisulfite sequencing, library preparation, and sequencing using their established protocols. ..

    Article Title: Establishment of a forward genetic screening system to discover new proteins associated with DNA demethylation pathway.
    Article Snippet: Active DNA demethylation mediated by Repressor of Silencing 1 (ROS1) plays a major role in counteracting gene silencing and spreading of DNA methylation.. However, the molecular mechanisms behind the ROS1-mediated DNA demethylation pathway remain largely unclear.. To gain a deeper understanding of this process, we established a novel HTC2-pUbq10-Ω-Kozak::LUC-1-based (H2UL-1-based) forward genetic screening system to identify more protein factors involved in such a pathway.

    Article Title: Centromeres are hotspots of cytosine methylation epimutations in a filamentous fungus
    Article Snippet: DNA samples were spiked with 1 ng of unmethylated λ -phage DNA (Ambion, USA) to monitor bisulfite conversion efficiency. .. For whole genome bisulfite sequencing, DNA samples were sent to Novogene (Cambridge, UK). ..

    Sequencing:

    Article Title: TIGAR deficiency enhances cardiac resilience through epigenetic programming of Parkin expression.
    Article Snippet: .. These samples were submitted to Novogene for whole genome bisulfite sequencing, library preparation, and sequencing using their established protocols. ..



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    Image Search Results


    Samples taken from the two MA pedigrees for different experiments. A) Ten MA lines were sampled from the two pedigrees. Samples for sequencing were taken after 5, 20, and 40 transfers and DNA methylation was detected using bisulfite sequencing (red circles). B) Three additional MA lines were sampled from both pedigrees and samples were taken after 1, 5, 7, 8, 10, and 15 transfers for Nanopore sequencing and after 5, 8, 10, 20, and 40 transfers for H3K9me3 ChIP-seq.

    Journal: bioRxiv

    Article Title: Centromeres are hotspots of cytosine methylation epimutations in a filamentous fungus

    doi: 10.64898/2026.03.03.709258

    Figure Lengend Snippet: Samples taken from the two MA pedigrees for different experiments. A) Ten MA lines were sampled from the two pedigrees. Samples for sequencing were taken after 5, 20, and 40 transfers and DNA methylation was detected using bisulfite sequencing (red circles). B) Three additional MA lines were sampled from both pedigrees and samples were taken after 1, 5, 7, 8, 10, and 15 transfers for Nanopore sequencing and after 5, 8, 10, 20, and 40 transfers for H3K9me3 ChIP-seq.

    Article Snippet: For whole genome bisulfite sequencing, DNA samples were sent to Novogene (Cambridge, UK).

    Techniques: Sequencing, DNA Methylation Assay, Methylation Sequencing, Nanopore Sequencing, ChIP-sequencing

    Pairwise divergence in cytosine methylation patterns among the MA lines and their ancestors. X-axis shows the number of mitoses separating the two lines that are being compared, and the y-axis shows cytosine methylation divergence. Red line shows a neutral epimutation accumulation model fitted to all data, orange line shows a neutral epimutation accumulation model fitted to data with outlier samples removed, and solid blue line shows a logistic model fitted to all data, and the dotted line shows null model of no increase in divergence. A) The divergence calculated from single cytosines, for CG, CHG, and CHH sequence motifs. B) The divergence calculated for DMRs in different sequence contexts, as in panel A.

    Journal: bioRxiv

    Article Title: Centromeres are hotspots of cytosine methylation epimutations in a filamentous fungus

    doi: 10.64898/2026.03.03.709258

    Figure Lengend Snippet: Pairwise divergence in cytosine methylation patterns among the MA lines and their ancestors. X-axis shows the number of mitoses separating the two lines that are being compared, and the y-axis shows cytosine methylation divergence. Red line shows a neutral epimutation accumulation model fitted to all data, orange line shows a neutral epimutation accumulation model fitted to data with outlier samples removed, and solid blue line shows a logistic model fitted to all data, and the dotted line shows null model of no increase in divergence. A) The divergence calculated from single cytosines, for CG, CHG, and CHH sequence motifs. B) The divergence calculated for DMRs in different sequence contexts, as in panel A.

    Article Snippet: For whole genome bisulfite sequencing, DNA samples were sent to Novogene (Cambridge, UK).

    Techniques: Methylation, Sequencing

    Whole-genome DNA methylation analysis of Chinese yam variant F2000 and F60. A Principal component 1 and principal component 2 analyses were performed for each sample. B Methylation level distribution across genetic features including 4 kb upstream of TSS, gene body, and 4 kb downstream of TTS in the three DNA methylation contexts CG, CHG, and CHH. Analyses were performed in biological triplicates for each variant. C Number of DMRs with higher methylation levels in F2000 (hypermethylated) and in F60 (hypomethylated). DMRs were investigated in the three DNA methylation contexts CG, CHG and CHH. Distribution of DMRs upstream in the putative promoter (4 kb of TSS), within the gene body, and down-stream in the putative terminator (4 kb of TTS) were analyzed in DMR-associated genes (q-value ≤ 0.01, methylation difference >|10%|). D Venn analysis of DMR-associated genes split in hyper- and hypomethylated promoters, gene bodies, and terminators. DMR, differentially methylated region; TSS, transcription start site; TTS, transcription termination site

    Journal: BMC Plant Biology

    Article Title: Insights into epigenetics suggest a role of DNA methylation in regulating Chinese yam tuber shape

    doi: 10.1186/s12870-026-08438-5

    Figure Lengend Snippet: Whole-genome DNA methylation analysis of Chinese yam variant F2000 and F60. A Principal component 1 and principal component 2 analyses were performed for each sample. B Methylation level distribution across genetic features including 4 kb upstream of TSS, gene body, and 4 kb downstream of TTS in the three DNA methylation contexts CG, CHG, and CHH. Analyses were performed in biological triplicates for each variant. C Number of DMRs with higher methylation levels in F2000 (hypermethylated) and in F60 (hypomethylated). DMRs were investigated in the three DNA methylation contexts CG, CHG and CHH. Distribution of DMRs upstream in the putative promoter (4 kb of TSS), within the gene body, and down-stream in the putative terminator (4 kb of TTS) were analyzed in DMR-associated genes (q-value ≤ 0.01, methylation difference >|10%|). D Venn analysis of DMR-associated genes split in hyper- and hypomethylated promoters, gene bodies, and terminators. DMR, differentially methylated region; TSS, transcription start site; TTS, transcription termination site

    Article Snippet: Prepared DNA samples were sent to Novogene Company (Beijing, China) for low-input whole-genome bisulfite sequencing including the steps of bisulfite treatment and library construction.

    Techniques: DNA Methylation Assay, Variant Assay, Methylation